Posters
2019
Kozak RP, Kotsias M, Hendel JL, Urbanowicz PA
Presented at: WCB 2019
Reston, USA. Nov 11-13th 2019
Targeted Analysis of Glycosylation Critical Quality Attributes from Glycoprotein Therapeutics
Urbanowicz PA, Hendel JL, Kotsias M, Kozak RP
Presented at: BioProduction 2019
Frankfurt, Germany. Nov 5-7th 2019
Strategic Implementation of Glycan Standards for Reliable Analysis of Glycomolecules
Hendel JL, Urbanowicz PA, Kozak RP
Presented at: XXth European Carbohydrate Symposium (EUROCARB2019)
Leiden, Netherlands. June 30th-July 4th 2019
Detection of HNF1A MODY diabetes with glycan biomarkers
Demus D, Urbanowicz PA, Gardner RA, Jansen BC, Wuhrer M, Owen KR, Spencer D
Presented at: XXth European Carbohydrate Symposium (EUROCARB2019)
Leiden, Netherlands. June 30th-July 4th 2019
Amez Martin M, Badía Tortosa C, Hendel JL, Gardner RA, Ledru H, Galan MC, Wuhrer M, Spencer D
Presented at: VI Symposium of Medicinal Chemistry Young Researchers
Madrid, Spain. June 21st 2019
and XXth European Carbohydrate Symposium (EUROCARB2019)
Leiden, Netherlands. June 30th-July 4th 2019
Serum N-glycomic biomarkers predict treatment escalation in Inflammatory Bowel Disease (IBD)
Shubhakar A, Jansen BC, Adams AT, Reiding KR, Ventham NT, Kalla R, Bergemalm D, Urbanowicz PA, Gardner RA, IBD BIOM Consortium, Halfvarson J, Satsangi J, Fernandes DL, Wuhrer M, Spencer D
Presented at: Digestive Diseases Week
San Diego, USA. May 18-21st 2019
2018
Kotsias M, Kozak RP, Gardner RA, Wuhrer M, Spencer D
Presented at: Society for GlycoBiology 2018 Annual Meeting
New Orleans, USA. November 5-8th 2018
Glycan Standards as Key Tools for Robust and Reliable Analysis of Glycoproteins
Hendel J, Peel S, Spencer D
Presented at: 29th International Carbohydrate Symposium
Lisbon, Portugal. July 15-19th 2018
Awarded the Chemical Biology Poster Prize by the Royal Society of Chemistry's 'Organic & Biomolecular Chemistry' Journal
Thomson RI, Gardner RA, Kozak RP, Smith J, Strohfeldt K, Osborn HMI, Spencer DI
Presented at: WCBP 2018: 22nd Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington D.C., United States. January 30-February 1st 2018
Sialylation: A Critical Glycosylation Quality Attribute for Biopharmaceuticals
Hendel JL, Kozak RP, Morgan CL, Royle L
Presented at: WCBP 2018: 22nd Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington D.C., United States. January 30-February 1st 2018
2017
Development of a High Throughput Method for
O-Glycosylation Analysis
Kotsias M, Gardner R, Kozak RP, Wuhrer M, Spencer D
Presented at: 12th Jenner Glycobiology and Medicine Symposium: Translational Glycobiology
Dubrovnik, Croatia. May 6-9th 2017
Keywords: Colorectal cancer (CRC), O-glycosylation, permethylation, MALDI-TOF-MS, high throughput analysis, GlycoCan
Analysis of Sialic Acids in Biopharmaceuticals
Hendel J, Royle L, Kozak RP, Fernandes DL
Presented at: WCBP 2017: 21st Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington D.C., United States. January 24-26th 2017
Keywords: RP-HPLC, EPO, Mabs, fetuin, sialic acid, critical quality attribute (CQA), quantitation, Neu5Ac, Neu5Gc, DMB
2016
Automated High-Throughput Permethylation for Glycosylation Analysis Using MALDI-TOF-MS
Shubhakar A, Spencer D, Wuhrer M, Fernandes DL
Presented at the 38th Annual Congress of Pharmacology Society of Chile
Castro, Chile. November 26-29th 2016
Keywords: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), permethylation, high throughput (HTP), N-glycans, O-glycans
Wongtrakul-Kish K, Kozak RP, Spencer D
Presented at the GlycoPar Symposium & Workshop
Liverpool, UK. September 12-14th 2016
Keywords: Procainamide, LC-MS, HILIC-HPLC-ESI-MS/MS, parasitic glycans, insect vector glycans, host glycans, GlycoPar
First steps in the development of a high throughput method for O-glycosylation analysis
Kotsias M, Kozak RP, Shubhakar A, Fernandes DL, Spencer D
Presented at the GlycoPar Symposium & Workshop
Liverpool, UK. September 12-14th 2016
Keywords: Colorectal cancer (CRC), O glycans, permethylation, MALDI-TOF-MS, GlycoCan
Spencer D, Smith J, Gardner R, Galvin B, Resemann A, Hufnagel P, Fernandes D
Presented at the 64th ASMS Conference:
American Society for Mass Spectrometry
San Antonio TX, United States. June 5-9th 2016
Keywords: erythropoietin (EPO), glycan, glycopeptide, LC-ESI-QTof-MS, ProteinScape, critical quality attribute (CQA)
Kozak RP, Royle L, Liew LP, Spencer DI, Fernandes DL
Presented at WCBP 2016: 20th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington DC, United States. January 2016
Keywords: Gal alpha 1-3 gal, IgG, monoclonal antibody (mAb), glycosylation critical quality attribute (GCQA), QbD, procainamide, UHPLC, ESI-MS/MS
Fernandes DL, Thomson R, Gardner R, Liew LP, Kozak RP, Royle L, Strohfeldt K, Osborn H, Spencer DI
Presented at WCBP 2016: 20th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington DC, United States. January 2016
Keywords: QbD, erythropoietin (EPO), sialate O-acetyl esterase (Nan S), glycosylation critical quality attribute (GCQA), sialic acids, DMB, procainamide, UHPLC, LC-MS
2015
Kozak RP, Wongtrakul-Kish K, Williams C, Fernandes DL, Perally S, Rose C, Spencer DI, Acosta-Serrano A
Presented at Glyco23: 23rd International Symposium on Glycoconjugates
Split, Croatia. September 2015
Keywords: trypanosomiasis, saliva, 2AB, procainamide, UHPLC, ESI-MS/MS
Hendel JL, Badía Tortosa C, Spencer DI, Fernandes DL
Presented at USP: Glycosylation Analysis for Biopharmaceuticals Workshop
Washington DC, United States. August 2015
Keywords: glycopeptide, IgG, monoclonal antibody (mAb), peptide mapping, V-Tag, UHPLC, MALDI-MS
Shubhakar A, Spencer DI, Wuhrer M, Fernandes DL
Presented at Gordon Research Conference
Lucca, Italy. March 2015
Kozak RP, Wongtrakul-Kish K, Williams C, Perally S, Rose C, Spencer DI, Acosta-Serrano A
Presented at:
Gordon Research Conference
Lucca, Italy. March 2015
and CASSS Analytical Technologies Conference
Berlin, Germany. March 2015
2014
Drug Glycosylation Analysis using Automated,
High Throughput, Orthogonal Methods
Shubhakar A, Kozak RP, Badía Tortosa C, Gardner RA, Royle L, Wuhrer M, Spencer DI, Fernandes DL
Presented at PEGS 2014: Glycosylation Analysis for Biopharmaceuticals Workshop
Boston, United States. May 2014
Keywords: high throughput, permethylation, V-Tag, glycosylation critical quality attribute (GCQA), IgG, erythropeitin (EPO), UHPLC, MALDI-MS
Studies on Salivary Glycosylation
Urbanowicz PA, Kozak RP, Fernandes DL
Presented at ACTA: 10th European Symposium on Saliva
Egmond aan Zee, Netherlands. May 2014
Keywords: saliva, O glycans, Orela, 2AB, glucose homopolymer standard, T1 cartridges, UHPLC, MALDI-MS
Shubhakar A, Spencer DI, Kozak RP, Velez-Suberbie L, Bracewell DG, Fernandes DL, Wuhrer M
Presented at ICS: 27th International Carbohydrate Symposium
Bangalore, India. January 2014
Keywords: high throughput, glycosylation critical quality attribute (GCQA), IgG4, bioreactor, permethylation, UHPLC, MALDI-MS
2013
Systematic Analysis of Drug Glycosylation Critical Quality Attributes (GCQAs)
Royle L, Kozak RP, Liew LP, Emery RJ, Fernandes DL
Presented at Glyco22: 22nd International Symposium on Glycoconjugates
Dalian, China. June 2013
2012
Suppression of Peeling during the Release of O-glycans by Hydrazinolysis
Kozak RP, Royle L, Gardner RA, Fernandes DL, Wuhrer M
Presented at the 4th Warren Workshop
Athens, Georgia, United States. August 2012
Badía Tortosa C, Fernandes DL, Spencer DI
Presented at:
International Glycomics Symposium
San Sebastian, Spain. July 19-21st 2012
and 26th International Carbohydrate Symposium (ICS)
Madrid, Spain. July 22-27th 2012