50mL of x4 LS-C buffer concentrate (2.0M ammonium formate buffer, pH 9.0). This can be diluted with water and acetonitrile to prepare LS-C solvent (500 mM ammonium formate buffer pH 9, 20% acetonitrile v/v) used in WAX (weak anion exchange) HPLC analysis of LudgerTag fluorophore and UV-chromophore labelled glycans.
50ml of x40 LS-N buffer concentrate (2.0M ammonium formate buffer, pH 4.4). This can be diluted with water to prepare LS-N buffer (50 mM ammonium formate buffer, pH 4.4) used in amide or HILIC (hydrophilic interaction liquid chromatography) HPLC analysis of LudgerTag fluorophore and UV-chromophore labelled glycans.