Quantitative Monosaccharide Release and 2-AA (2-aminobenzoic acid) Labelling Kit

Monosaccharide analysis is a regulatory requirement laid out in the ICH Q6B guidelines for characterisation of biopharmaceuticals. This information can be used at all stages of drug development as a method of determining the type of glycosylation (N-linked and/or O-linked) and the extent to which glycosylation has occurred. It can also be used to demonstrate consistency between batches for QC lot release during the manufacturing process.

Ludger’s monosaccharide analysis kit is easy to use, reliable and can be used for quantitative routine monosaccharide analysis. (Kit sufficient for 96 analyses).

• Release neutral and amino monosaccharides from your glycoprotein samples by mild acid hydrolysis
• Fluorescent labelling of released monosaccharides with 2-aminobenzoic acid (2AA)
• Relative quantitative analysis of 2AA-labelled monosaccharides compared to standards (GlcN, GalN, Gal, Man, Glc, Fuc, Xyl) by (U)HPLC analysis

• Info Guides: Monosaccharide Analysis Guide

• Product Guide: LT-MONO-96

Product/Ordering Information:

Release monosaccharides and label with 2AA:

• LT-MONO-96
• LudgerTag Monosaccharide Release and Labelling Kit

Each kit contains reagents and materials for up to 96 glycoprotein samples, control and standards analysed in parallel or two sets of 48 samples:

• Trifluoroacetic acid (LT-2MTFA-01 - 2 vials)
• Hydrochloric acid (LT-6MHCL-01 - 2 vials)
• Sodium acetate (LT-NAOAC-01 - 2 vials)
• Labelling solvent (LT-NBM-01 - 2 vials)
• 2-aminobenzoic acid (LT-2AA-02 - 2 vials)
• Sodium cyanoborohydride (LT-CYANOB-03 - 2 vials)
• 10 nmols each of glucosamine (GlcN), galactosamine (GalN), 4 Galactose (Gal), glucose (Glc), mannose (Man) and fucose (Fuc) (CM-MONO-MIX-10 - 4 vials)
• 100 nmol of xylose (Xyl) (CM-XYL-100 - 2 vials)

Companion Products:

Positive Control:

• GCP-FET-50U-X4
• Fetuin Glycoprotein Standard (4 vials containing 50µg)
• BQ-GPEP-A2G2S2-10U
• LudgerBioQuant GPEP A2G2S2 Glycopeptide Standard

Standards:

• CM-MONOMIX-10
• Ludger Quantitative Monosaccharide Standard (contained in LT-MONO-96 kit - additional standard available for purchase)
• CM-XYLOSE-100
• Ludger Xylose Standard (contained in LT-MONO-96 kit - additional standard available for purchase)

HPLC Analysis:

• LS-R2-4.6x150
• LudgerSep R2 HPLC Column

UHPLC Analysis:

• LS-UR2-2.1x50
• LudgerSep uR2 UHPLC Column

Solvent:

• LS-R-BPTX10
• LudgerSep R BPT solvent (x10 concentrate)