Glycan Analysis Services

Intact Released Glycan Analysis



Ludger Intact Released Glycan Analysis



Presentation Quick Links:

  1. Glycan Analysis and why it is important

  2. Analysis of Intact Released Glycans: Overview

  3. Releasing Glycans: How to choose a method

  4. Glycan Analytical Methods and What Information is Obtained

  5. What Level of Information is Required?

  6. Detailed Characterisation Workflow for IgG N-Glycans

  7. Detailed Characterisation Workflow for EPO N-Glycans

  8. Examples of QC Analysis Methods for IgG GCQAs

  9. Examples of QC Analysis Methods for EPO GCQAs



Relevant Posters

Ludger poster

Biopharmaceutical Erythropoietin Characterisation: critical quality attribute (CQA) mapping using LC-ESI-Qtof and automated database searching of glycopeptide analytes

Spencer D, Smith J, Gardner R, Galvin B, Resemann A, Hufnagel P, Fernandes D
Presented at the 64th ASMS Conference: American Society for Mass Spectrometry
San Antonio TX, United States. June 5-9th 2016

Keywords: erythropoietin (EPO), glycan, glycopeptide, LC-ESI-QTof-MS, ProteinScape, critical quality attribute (CQA)



Ludger poster

Procainamide labelling as part of a flexible glycoprofiling system for monitoring of Gal-a1-3Gal related Glycosylation Critical Quality Attributes (GCQAs) of monoclonal antibody (mAb) therapeutics throughout the product life cycle

Kozak RP, Royle L, Liew LP, Spencer DI, Fernandes DL
Presented at WCBP 2016: 20th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington DC, United States. January 2016

Keywords: Gal alpha 1-3 gal, IgG, monoclonal antibody (mAb), glycosylation critical quality attribute (GCQA), QbD, procainamide, UHPLC, ESI-MS/MS



Ludger poster

A QbD-compatible approach for reliable measurement of sialic acid O-acetylation as a potential Glycosylation Critical Quality Attribute (GCQA) of erythropoietin (EPO) therapeutics

Fernandes DL, Thomson R, Gardner R, Liew LP, Kozak RP, Royle L, Strohfeldt K, Osborn H, Spencer DI
Presented at WCBP 2016: 20th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington DC, United States. January 2016

Keywords: QbD, erythropoietin (EPO), sialate O-acetyl esterase (Nan S), glycosylation critical quality attribute (GCQA), sialic acids, DMB, procainamide, UHPLC, LC-MS