Publications


2018



Transcriptional activation of fucosyltransferase (FUT) genes using the CRISPR-dCas9-VPR technology reveals potent N-glycome alterations in colorectal cancer cells.

Blanas A, Cornelissen LAM, Kotsias M, van der Horst JC, van de Vrugt HJ, Kalay H, Spencer DIR, Kozak RP, van Vliet SJ. Glycobiology. 2018 Nov 22. doi: 10.1093/glycob/cwy096. [Epub ahead of print]




High-throughput serum N-glycomics: method comparison and application to study rheumatoid arthritis and pregnancy-associated changes.

Reiding KR, Bondt A, Hennig R, Gardner RA, O'Flaherty R, Trbojevic-Akmacic I, Shubhakar A, Hazes J, Reichl U, Fernandes DL, Pucic-Bakovic M, Rapp E, Spencer DIR, Dolhain R, Rudd P, Lauc G, Wuhrer M. Mol Cell Proteomics. 2018 Sep 21. pii: mcp.RA117.000454. doi: 10.1074/mcp.RA117.000454




GlycoStore: a database of retention properties for glycan analysis.

Zhao S, Walsh I, Abrahams JL, Royle L, Nguyen-Khuong T, Spencer D, Fernandes DL, Packer NH, Rudd PM, Campbell MP. Bioinformatics. 2018 Sep 15;34(18):3231-3232. doi: 10.1093/bioinformatics/bty319.




HappyTools: A software for high-throughput HPLC data processing and quantitation.

Jansen BC, Hafkenscheid L, Bondt A, Gardner RA, Hendel JL, Wuhrer M, Spencer DIR. PLoS One. 2018 Jul 6;13(7):e0200280. doi: 10.1371/journal.pone.0200280. eCollection 2018.




Towards automation of glycomic profiling of complex biological materials.

Shubhakar A, Pang PC, Fernandes DL, Dell A, Spencer DIR, Haslam SM. Glycoconj J. 2018 Jun 16. doi: 10.1007/s10719-018-9825-8. [Epub ahead of print]




Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncology.

Crescioli S, Chiaruttini G, Mele S, Ilieva KM, Pellizzari G, Spencer DIR, Gardner RA, Lacy KE, Spicer JF, Tutt ANJ, Wagner GK, Karagiannis SN. J Allergy Clin Immunol. 2018 Apr;141(4):1519-1523.e9. doi: 10.1016/j.jaci.2017.12.986. Epub 2018 Jan 31. No abstract available.



2017



Analysis of Three Epoetin Alpha Products by LC and LC-MS Indicates Differences in Glycosylation Critical Quality Attributes, Including Sialic Acid Content.

Thomson RI, Gardner RA, Strohfeldt K, Fernandes DL, Stafford GP, Spencer DIR, Osborn HMI. Anal Chem. 2017 Jun 20;89(12):6455-6462. doi: 10.1021/acs.analchem.7b00353. Epub 2017 Jun 9.



2016



Variation of Human Salivary O-Glycome.

Kozak RP, Urbanowicz PA, Punyadeera C, Reiding KR, Jansen BC, Royle L, Spencer DI, Fernandes DL, Wuhrer M. PLoS One. 2016 Sep 9;11(9):e0162824. doi: 10.1371/journal.pone.0162824. eCollection 2016.




Automated High-Throughput Permethylation for Glycosylation Analysis of Biologics Using MALDI-TOF-MS.

Shubhakar A, Kozak RP, Reiding KR, Royle L, Spencer DI, Fernandes DL, Wuhrer M. Anal Chem. 2016 Sep 6;88(17):8562-9. doi: 10.1021/acs.analchem.6b01639. Epub 2016 Aug 15.




Composition and Antigenic Effects of Individual Glycan Sites of a Trimeric HIV-1 Envelope Glycoprotein.

Behrens AJ, Vasiljevic S, Pritchard LK, Harvey DJ, Andev RS, Krumm SA, Struwe WB, Cupo A, Kumar A, Zitzmann N, Seabright GE, Kramer HB, Spencer DI, Royle L, Lee JH, Klasse PJ, Burton DR, Wilson IA, Ward AB, Sanders RW, Moore JP, Doores KJ, Crispin M. Cell Rep. 2016 Mar 22;14(11):2695-706. doi: 10.1016/j.celrep.2016.02.058. Epub 2016 Mar 10.




Longitudinal monitoring of immunoglobulin A glycosylation during pregnancy by simultaneous MALDI-FTICR-MS analysis of N- and O-glycopeptides.

Bondt A, Nicolardi S, Jansen BC, Stavenhagen K, Blank D, Kammeijer GS, Kozak RP, Fernandes DL, Hensbergen PJ, Hazes JM, van der Burgt YE, Dolhain RJ, Wuhrer M. Sci Rep. 2016 Jun 15;6:27955. doi: 10.1038/srep27955.



2015



Characterization of a sialate-O-acetylesterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid release by NanH, its cognate sialidase.

Phansopa C, Kozak RP, Liew LP, Frey AM, Farmilo T, Parker JL, Kelly DJ, Emery RJ, Thomson RI, Royle L, Gardner RA, Spencer DI, Stafford GP. Biochem J. 2015 Dec 1;472(2):157-67. doi: 10.1042/BJ20150388. Epub 2015 Sep 16.




Parasite Glycobiology: A Bittersweet Symphony.

Rodrigues JA, Acosta-Serrano A, Aebi M, Ferguson MA, Routier FH, Schiller I, Soares S, Spencer D, Titz A, Wilson IB, Izquierdo L. PLoS Pathog. 2015 Nov 12;11(11):e1005169. doi: 10.1371/journal.ppat.1005169. eCollection 2015. Review. No abstract available.




Comparison of procainamide and 2-aminobenzamide labeling for profiling and identification of glycans by liquid chromatography with fluorescence detection coupled to electrospray ionization-mass spectrometry.

Kozak RP, Tortosa CB, Fernandes DL, Spencer DI. Anal Biochem. 2015 Oct 1;486:38-40. doi: 10.1016/j.ab.2015.06.006. Epub 2015 Jun 12.




Glycan Microheterogeneity at the PGT135 Antibody Recognition Site on HIV-1 gp120 Reveals a Molecular Mechanism for Neutralization Resistance.

Pritchard LK, Spencer DI, Royle L, Vasiljevic S, Krumm SA, Doores KJ, Crispin M. J Virol. 2015 Jul;89(13):6952-9. doi: 10.1128/JVI.00230-15. Epub 2015 Apr 15.




Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies.

Pritchard LK, Spencer DI, Royle L, Bonomelli C, Seabright GE, Behrens AJ, Kulp DW, Menis S, Krumm SA, Dunlop DC, Crispin DJ, Bowden TA, Scanlan CN, Ward AB, Schief WR, Doores KJ, Crispin M. Nat Commun. 2015 Jun 24;6:7479. doi: 10.1038/ncomms8479.




Development of a Schistosoma mansoni shotgun O-glycan microarray and application to the discovery of new antigenic schistosome glycan motifs.

van Diepen A, van der Plas AJ, Kozak RP, Royle L, Dunne DW, Hokke CH.
Int J Parasitol. 2015 Jun;45(7):465-75. doi: 10.1016/j.ijpara.2015.02.008. Epub 2015 Mar 26.




Changes to serum sample tube and processing methodology does not cause Intra-Individual [corrected] variation in automated whole serum N-glycan profiling in health and disease.

Ventham NT, Gardner RA, Kennedy NA, Shubhakar A, Kalla R, Nimmo ER; IBD-BIOM Consortium, Fernandes DL, Satsangi J, Spencer DI.
PLoS One. 2015 Apr 1;10(4):e0123028. doi: 10.1371/journal.pone.0123028. eCollection 2015. Erratum in: PLoS One. 2015;10(6):e0129335.




High-Throughput Analysis and Automation for Glycomics Studies.

Shubhakar A, Reiding KR, Gardner RA, Spencer DI, Fernandes DL, Wuhrer M.
Chromatographia. 2015;78(5-6):321-333. Epub 2014 Nov 16. Review.




Mass spectrometry for glycosylation analysis of biopharmaceuticals.

Dotz V, Haselberg R, Shubhakar A, Kozak RP, Falck D, Rombouts Y, Reusch D, Somsen GW, Fernandes DL, Wuhrer M.
TrAC Trends in Analytical Chemistry. Volume 73, November 2015, Pages 1-9.



2014



The role of glycosylation in IBD.

Theodoratou E, Campbell H, Ventham NT, Kolarich D, Pučić-Baković M, Zoldoš V, Fernandes D, Pemberton IK, Rudan I, Kennedy NA, Wuhrer M, Nimmo E, Annese V, McGovern DP, Satsangi J, Lauc G.
Nat Rev Gastroenterol Hepatol. 2014 Oct;11(10):588-600. doi: 10.1038/nrgastro.2014.78. Epub 2014 Jun 10. Review.




Carbohydrate sequence of the prostate cancer-associated antigen F77 assigned by a mucin O-glycome designer array.

Gao C, Liu Y, Zhang H, Zhang Y, Fukuda MN, Palma AS, Kozak RP, Childs RA, Nonaka M, Li Z, Siegel DL, Hanfland P, Peehl DM, Chai W, Greene MI, Feizi T.
J Biol Chem. 2014 Jun 6;289(23):16462-77. doi: 10.1074/jbc.M114.558932. Epub 2014 Apr 21.




Improved nonreductive O-glycan release by hydrazinolysis with ethylenediaminetetraacetic acid addition.

Kozak RP, Royle L, Gardner RA, Bondt A, Fernandes DL, Wuhrer M.
Anal Biochem. 2014 May 15;453:29-37. doi: 10.1016/j.ab.2014.02.030. Epub 2014 Mar 5.




Mutations in B4GALNT1 (GM2 synthase) underlie a new disorder of ganglioside biosynthesis.

Harlalka GV, Lehman A, Chioza B, Baple EL, Maroofian R, Cross H, Sreekantan-Nair A, Priestman DA, Al-Turki S, McEntagart ME, Proukakis C, Royle L, Kozak RP, Bastaki L, Patton M, Wagner K, Coblentz R, Price J, Mezei M, Schlade-Bartusiak K, Platt FM, Hurles ME, Crosby AH.
Brain. 2013 Dec;136(Pt 12):3618-24. doi: 10.1093/brain/awt270. Epub 2013 Oct 7.



2013



Effect of a tail piece cysteine deletion on biochemical and functional properties of an epidermal growth factor receptor-directed IgA2m(1) antibody.

Brunke C, Lohse S, Derer S, Peipp M, Boross P, Kellner C, Beyer T, Dechant M, Royle L, Liew LP, Leusen JH, Valerius T.
MAbs. 2013 Nov-Dec;5(6):936-45. doi: 10.4161/mabs.26396.



2012



Suppression of peeling during the release of O-glycans by hydrazinolysis.

Kozak RP, Royle L, Gardner RA, Fernandes DL, Wuhrer M.
Anal Biochem. 2012 Apr 1;423(1):119-28. doi: 10.1016/j.ab.2012.01.002. Epub 2012 Jan 20.




Protein O-glycosylation analysis.

Zauner G, Kozak RP, Gardner RA, Fernandes DL, Deelder AM, Wuhrer M.
Biol Chem. 2012 Aug;393(8):687-708. doi: 10.1515/hsz-2012-0144. Review.