Ludger Sialic Acid Analysis


Quantitative Sialic Acid Release and DMB Labeling Kit

Sialic acid analysis workflow designed to satisfy the regulatory requirements for biopharmaceutical sialic acid content analysis to specify the following:

  1. Overall degree of drug sialylation - absolute quantitation of sialic acid residues per molecule (nmol/mg protein)
  2. Relative quantities of Neu5Ac:Neu5Gc
  3. Identification and relative percentage of O-acetylated sialic acids




  Product/Ordering Information:

Release and label sialic acids with DMB:

  • LudgerTag DMB sialic acid release and labeling kit



Ludger Quantitative Sialic Acid Release and DMB Labeling Kit

Each kit is suitable for analysing 22 samples, and contains the following:

  • DMB Dye  (LT-DMB-01)
  • Acetic Acid 2 Molar  (LT-ACETIC2M-01)
  • Mercaptoethanol in Acetic acid  (LT-MERCAPTO-01)
  • Sodium Dithionite (Reductant)  (LT-DITHIO-01)
  • N -acetylneuraminic acid (Neu5Ac or NANA) -- Quantitative Standard  (CM-NEU-AC-01)
  • N -glycolylneuraminic acid (Neu5Gc or NGNA) -- Quantitative Standard  (CM-NEU-GC-01)
  • Sialic Acid Reference Panel (containing Neu5Ac, Neu5Gc, Neu5,7Ac2, Neu5Gc,9Ac, Neu5,8Ac2, Neu5,9Ac2 and Neu5,x,xAc3 (where x is an unknown acetyl position))
    (CM-SRP-01)

Companion Products:

Standards and Controls:

  • Fetuin Glycoprotein -- Process Standard
  • LudgerBioQuant GPEP A2G2S2 glycopeptide -- Quantitative Process Standard
  • 5-N -acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2) -- Reference Standard
  • N -acetylneuraminic acid (Neu5Ac or NANA) -- Quantitative Standard
  • N -glycolylneuraminic acid (Neu5Gc or NGNA) -- Quantitative Standard
  • Sialic acid reference panel -- System Suitability and Reference Standard













HPLC Analysis:

  • LudgerSep R1 HPLC Column (hydrophobic chromatography column containing particles with an octadecylsilane coating)



UHPLC Analysis:

  • LudgerSep uR2 UHPLC Column (hydrophobic chromatography column containing particles with an octadecylsilane coating)







Posters

Ludger ICS 2018 Glycan Standards poster

Glycan Standards as Key Tools for Robust and Reliable Analysis of Glycoproteins

Hendel J, Peel S, Spencer D
Presented at: 29th International Carbohydrate Symposium
Lisbon, Portugal. July 15-19th 2018

Awarded the Chemical Biology Poster Prize by the Royal Society of Chemistry's 'Organic & Biomolecular Chemistry' Journal












Ludger WCBP2018 Sialylation poster

Sialylation: A Critical Glycosylation Quality Attribute for Biopharmaceuticals

Hendel JL, Kozak RP, Morgan CL, Royle L
Presented at: WCBP 2018: 22nd Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington D.C., United States. January 30-February 1st 2018






WCBP Sialic Acid Poster

Analysis of Sialic Acids in Biopharmaceuticals

Hendel J, Royle L, Kozak RP, Fernandes DL

Presented at:
WCBP 2017: 21st Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington D.C., United States. January 24-26th 2017















Contacts

Technical Advice

Dr. Jenifer Hendel

Dr. Jenifer Hendel
Senior Scientist
jenifer.hendel@ludger.com

Quotations and Orders

Ludger DMB Sialic Acid Release and Glycan Labeling Kit

Sales Team
Quotations: info@ludger.com
Orders: sales@ludger.com