Ludger O-glycosidase enzyme structure

    O-Glycosidase


    E-G001

    Cleaves only unsubstituted Gal-β(1-3)GalNAc-α disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.

      View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-G001
    • Amount of Enzyme
    • 75 mU / 60 µL








    Kit includes enzyme plus reaction buffer.
    Sufficient for up to 60 reactions.






    Product Specifications:


    Specificity:
    O-glycosidase leaves only unsubstituted Gal-β(1-3)GalNAc-α disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.

    Substitutions such as sialic acid, galactose, fucose or N-acetylglucosamine must first be removed with the appropriate exoglycosidase prior to treatment with Endo-O-Glycosidase. At minimum, a sialadase such as Sialadase Au α(2-3,6,8,9), part number E-S001, is almost always required to remove sialic acids.

    Source: Recombinant Streptococcus pneumoniae in E.Coli

    EC: 3.2.1.97

    Contents:
    Ludger O-Glycosidase - Kit contents
    60 µL aliquot of enzyme (75 mU) in 50 mM sodium phosphate, pH 7.5.
    5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)

    Specific Activity: >12 U/mg

    Activity: >1.25 U/mL

    Molecular weight: 180,000 daltons

    pH range: 5-7, optimum 5.0

    Suggested usage:
    1. Add up to 100 µg of glycoprotein to a tube.
    2. Add de-ionized water to a total of 13 µL.
    3. Add 4 µL of 5x Reaction Buffer 5.0.
    4. Add 1 µL of Sialadase Au α(2-3,6,8,9), part number E-S001.
    5. Add 2 µL of O-Glycosidase.
    6. Incubate for 1 hour at 37°C.

    Specific Activity: Defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C, pH 5.0 from p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside.

    Storage: Store enzyme at 4°C.

    Stability: Stable at least 12 months when stored properly. Several days exposure to ambient temperature will not reduce activity. Active for at lease 5 days under reaction conditions.

    Purity: O-Glycosidase is tested for contaminating protease as follows; 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.





    References:

    1. Bhavanandan, V.P., J. Umemoto and E.A. Davidson. Characterization of an endo-alpha-N-acetylgalactosaminidase from Diplococcus pneumoniae. Biochem Biophys 70: 738-74 5 (1976 ).

    2. Fan, J.Q., K. Yamamoto, H. Kumagai and T. Tochikura. Induction and efficient purification of endo-alpha-N-acetyl- D-galactosaminidase from Alcaligenes sp. Agric Biol Chem 54: 233-23 4 (1990 ).

    3. Glasgow, L R., J.C. Paulson and R.L. Hill. Systematic purification of five glycosidases from Streptococcus pneumoniae. J Biol Chem 252: 8615-8 623 (1977).

    4. Iwase, H. and K . Hotta. Release of O-linked glycoprotein glycans by endo-alpha-N-acetyl-D-galactosaminidase. Meth Mol Biol 14: 151-159 (1993).

    5. Unemoto, J., V.P. Bhavanandan and E.A. Davidson. Purification and properties of an endo-alpha-N-acetyl-D-galactosaminidase from Diplococcus pneumoniae. J Biol Chem 252: 8609-8 614 (1977).