Endoglycosidases:

Endoglycosidases are enzymes that cleave intact glycans from glycoproteins. These enzymes are highly stable in clean preparations with no glycerol, NaCl, or other additives such as EDTA. All enzymes are tested for the absence of proteolytic or unexpected glycosidic activity.

Endoglycosidases are some of the most widely used enzymes for the analysis of glycoproteins. These enzymes release intact glycans from glycoproteins, while exoglycosidases release individual monosaccharides (i.e. sialic acid, galactose, β-N-acetylgucosamine, mannose, etc.).

PNGase F is commonly included in the group of enzymes due to its similar activity of cleaving intact N-linked glycans, though it is actually an amidase. It cleaves between the asparagine amino acid and the first β-N-acetylglucoseamine sugar (GlcNAc) of the chitobiose core of N-linked glycans. The Endo F enzymes and Endo H cleave N-linked glycans between the first and second GlcNAc, leaving a charged residue which aids in increasing solubility of the deglycosylated protein, reducing the potential for precipitation of the protein aggregates. PNGase F cleaves all N-linked glycans, while Endo H and the Endo F enzymes remove specific types of N-linked glycans.

Also included in this classification of enzymes is O-Glycosidase, which removes the Core 1 O-Linked glycan (Gal-β(1-3)GalNAc-α) from serine or threonine amino acids. Often times O-linked glycans contain additional monosaccharides linked to the galactose in both branched and linear linkages, requiring the use of exoglycosidases such as sialidase and galactosidase to remove the additional sugars.

PNGase F (Peptide N Glycosidase F) and recombinant PNGase F


PNGase F is suitable for release of all types (high-mannose, hybrid and complex) N-linked glycans from glycoproteins and glycopeptides. PNGase F will not remove oligosaccharides containing α(1-3) linked core fucose commonly found on plant glycoproteins.

Table below lists and compares the PNGase F enzyme kits offered at Ludger.

Ludger Enzymes PNGaseF table
Ludger PNGase F enzyme structure

LZ-rPNGaseF-kit

LudgerZyme recombinant PNGase F kit (New England BioLabs).

From Elizabethkingia miricola in E. coli

Product guide

  • Part Number
  • LZ-rPNGaseF-kit
  • Amount of Enzyme
  • 150 µL

Kit includes enzyme plus reaction buffers.
Sufficient for up to 150 reactions.


E-PNG01

From Elizabethkingia meningosepticum

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-PNG01
  • Amount of Enzyme
  • 0.3 U / 60 µL

Kit includes enzyme plus reaction buffers.
Sufficient for up to 60 reactions.


E-PNG01-200      *formerly E-PNG05

From Elizabethkingia meningosepticum

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-PNG01-200
  • Amount of Enzyme
  • 1 U / 200 µL

Includes enzyme only.
Sufficient for up to 200 reactions.


E-rPNG01

Recombinant PNGase F from Elizabethkingia miricola.

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-rPNG01
  • Amount of Enzyme
  • 0.3 U / 60 µL

Kit includes enzyme plus reaction buffers.
Sufficient for up to 60 reactions.


Endoglycosidase F1


Ludger Endo F1 enzyme structure

E-EF01

Endo F1 cleaves high mannose and some hybrid type N-glycans from peptides and proteins.

Recombinant gene from Elizabethkingia miricola in E. Coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-EF01
  • Amount of Enzyme
  • 1 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


Endoglycosidase F2


Ludger Endo F2 enzyme structure

E-EF02

Selective release of biantennary and high mannose (at a 40X reduced rate) glycans from peptides and proteins.

Recombinant gene from Elizabethkingia miricola in E. Coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-EF02
  • Amount of Enzyme
  • 0.3 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


Endoglycosidase F3


Ludger Endo F3 enzyme structure

E-EF03

Selective release of triantennarry and α(1-6) fucosylated biantennary N-glycans from peptides and protein.

Recombinant gene from Elizabethkingia miricola in E. Coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-EF03
  • Amount of Enzyme
  • 0.33 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


Endoglycosidase H


Ludger Endo H enzyme structure

E-EHO2

Endo H cleaves asparagine-linked hybrid or high mannose oligosaccharides but not complex oligosaccharides.

Recombinant gene from Streptomyces plicatus in E. Coli

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-EH02
  • Amount of Enzyme
  • 0.3 U / 60 µL

Kit includes enzyme plus reaction buffers.
Sufficient for up to 60 reactions.


O-glycosidase


Ludger O-glycosidase enzyme structure

E-G001

Cleaves only unsubstituted Gal-β(1-3)GalNAc-α disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.

Recombinant from Streptococcus pneumoniae in E. Coli.

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-G001
  • Amount of Enzyme
  • 75 mU / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


Enzymatic CarboRelease Kit


Ludger Enzymatic Release enzyme structure

KE-DG01

This kit includes the enzymes and buffers required to deglycosylate glycoproteins, removing all N-linked oligosaccharides and many O-linked sugars.

The enzymes are packaged separately in individual 20 microlitre vials. This gives researchers the flexibility to characterize the glycans attached to their glycoprotein more fully than with our mixture of enzymes, KE-DGMX.

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • KE-DG01
  • Amount of Enzyme
  • 20 µL each enzyme

20 µL of each of the following enzymes in separate vials: PNGase F (Elizabethkingia meningosepticum), O-Glycosidase (Streptococcus pneumoniae), Sialidase (Arthrobacter ureafaciens), β-Galactosidase (Streptococcus pneumoniae), Glucosaminidase (Streptococcus pneumonia).

Kit includes enzyme, reaction buffers and Bovine Fetuin (control).
Each enzyme sufficient for up to 20 reactions.


Enzymatic DeGlycoMx Kit


Ludger Enzymatic Release enzyme structure

KE-DGMX

This kit developed for protein deglycosylation includes our DeGlycoMx, a premixed cocktail of the enzymes required to remove all N-linked oligosaccharides and most O-linked sugars from 0.5 mg of glycoprotein, via 10 reactions of up to 50 micrograms of protein per reaction.

The kit is easy-to-use and effective: add 2 µL of the DeGlycoMx enzyme to your denatured sample and the included buffer and incubate for 3 hours at 37°C.

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • KE-DGMX
  • Amount of Enzyme
  • 20 µL premix

The enzymes are provided as one 20 µL premixed cocktail including: PNGase F (Elizabethkingia meningosepticum), O-Glycosidase (Streptococcus pneumoniae), Sialidase (Arthrobacter ureafaciens), β-Galactosidase (Streptococcus pneumoniae), Glucosaminidase (Streptococcus pneumoniae).

Kit includes enzyme plus reaction buffers.
Sufficient for up to 20 reactions.


Endo-β-Galactosidase


Ludger Endo-beta-galactosidase enzyme structure

E-XBG01

Endo-β-galactosidase cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures.

Recombinant gene from Bacteroides fragilis in E. Coli.

View product documentation: Specsheet / CofA / MSDS

  • Part Number
  • E-XBG01
  • Amount of Enzyme
  • 0.9 U / 60 µL

Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.


LudgerZyme Ceramide Glycanase Kit


Ludger Ceramide Glycanase enzyme structure

LZ-CER-HM-KIT

Ceramide glycanase deglycosylates a variety of glycosphingolipids by cleaving the ß-glycosyl linkage.

From Hirudo medicinalis

Product overview

Product guide

Certificate of stability

  • Part Number
  • LZ-CER-HM-KIT
  • Amount of Enzyme
  • 55 µL

Kit includes enzyme, reaction buffer and GM1 standard.
Sufficient for up to 25 reactions.