Endoglycosidases:

Endoglycosidases are enzymes that cleave intact glycans from glycoproteins. These enzymes are highly stable in clean preparations with no glycerol, NaCl, or other additives such as EDTA. All enzymes are tested for the absence of proteolytic or unexpected glycosidic activity.

Endoglycosidases are some of the most widely used enzymes for the analysis of glycoproteins. These enzymes release intact glycans from glycoproteins, while exoglycosidases release individual monosaccharides (i.e. sialic acid, galactose, β-N-acetylgucosamine, mannose, etc.).

PNGase F is commonly included in the group of enzymes due to its similar activity of cleaving intact N-linked glycans, though it is actually an amidase. It cleaves between the asparagine amino acid and the first β-N-acetylglucoseamine sugar (GlcNAc) of the chitobiose core of N-linked glycans. The Endo F enzymes and Endo H cleave N-linked glycans between the first and second GlcNAc, leaving a charged residue which aids in increasing solubility of the deglycosylated protein, reducing the potential for precipitation of the protein aggregates. PNGase F cleaves all N-linked glycans, while Endo H and the Endo F enzymes remove specific types of N-linked glycans.

Also included in this classification of enzymes is O-Glycosidase, which removes the Core 1 O-Linked glycan (Gal-β(1-3)GalNAc-α) from serine or threonine amino acids. Often times O-linked glycans contain additional monosaccharides linked to the galactose in both branched and linear linkages, requiring the use of exoglycosidases such as sialidase and galactosidase to remove the additional sugars.


  • PNGase F (Peptide N Glycosidase F) and recombinant PNGase F



    PNGase F is suitable for release of all types (high-mannose, hybrid and complex) N-linked glycans from glycoproteins and glycopeptides. PNGase F will not remove oligosaccharides containing α(1-3) linked core fucose commonly found on plant glycoproteins.


    Table below lists and compares the PNGase F enzyme kits offered at Ludger.


    Ludger Enzymes PNGaseF table








    Ludger PNGase F enzyme structure

    LZ-rPNGaseF-kit

    LudgerZyme recombinant PNGase F kit (New England BioLabs).

    From Elizabethkingia miricola in E. coli

    Product guide

    CofA:   Batch # B7CC-01   Batch # B921-01

    • Part Number
    • LZ-rPNGaseF-kit
    • Amount of Enzyme
    • 150 µL






    Kit includes enzyme plus reaction buffers.

    Sufficient for up to 150 reactions.




    E-PNG01

    From Elizabethkingia meningosepticum

    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-PNG01
    • Amount of Enzyme
    • 0.3 U / 60 µL






    Kit includes enzyme plus reaction buffers.

    Sufficient for up to 60 reactions.




    E-PNG01-200    *formerly E-PNG05

    From Elizabethkingia meningosepticum

    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-PNG01-200
    • Amount of Enzyme
    • 1 U / 200 µL






    Includes enzyme only.

    Sufficient for up to 200 reactions.




    E-rPNG01

    Recombinant PNGase F from Elizabethkingia miricola.

    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-rPNG01
    • Amount of Enzyme
    • 0.3 U / 60 µL






    Kit includes enzyme plus reaction buffers.

    Sufficient for up to 60 reactions.


  • Endoglycosidase F1


    Ludger Endo F1 enzyme structure

    E-EF01

    Endo F1 cleaves high mannose and some hybrid type N-glycans from peptides and proteins.

    Recombinant gene from Elizabethkingia miricola in E. Coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-EF01
    • Amount of Enzyme
    • 1 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • Endoglycosidase F2



    Ludger Endo F2 enzyme structure

    E-EF02

    Selective release of biantennary and high mannose (at a 40X reduced rate) glycans from peptides and proteins.

    Recombinant gene from Elizabethkingia miricola in E. Coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-EF02
    • Amount of Enzyme
    • 0.3 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • Endoglycosidase F3



    Ludger Endo F3 enzyme structure

    E-EF03

    Selective release of triantennarry and α(1-6) fucosylated biantennary N-glycans from peptides and protein.

    Recombinant gene from Elizabethkingia miricola in E. Coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-EF03
    • Amount of Enzyme
    • 0.33 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • Endoglycosidase H



    Ludger Endo H enzyme structure

    E-EHO2

    Endo H cleaves asparagine-linked hybrid or high mannose oligosaccharides but not complex oligosaccharides.

    Recombinant gene from Streptomyces plicatus in E. Coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-EH02
    • Amount of Enzyme
    • 0.3 U / 60 µL






    Kit includes enzyme plus reaction buffers.

    Sufficient for up to 60 reactions.


  • O-glycosidase



    Ludger O-glycosidase enzyme structure

    E-G001

    Cleaves only unsubstituted Gal-β(1-3)GalNAc-α disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.

    Recombinant from Streptococcus pneumoniae in E. Coli.


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-G001
    • Amount of Enzyme
    • 75 mU / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • Enzymatic CarboRelease Kit



    Ludger Enzymatic Release enzyme structure

    KE-DG01

    This kit includes the enzymes and buffers required to deglycosylate glycoproteins, removing all N-linked oligosaccharides and many O-linked sugars.

    The enzymes are packaged separately in individual 20 microlitre vials. This gives researchers the flexibility to characterize the glycans attached to their glycoprotein more fully than with our mixture of enzymes, KE-DGMX.


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • KE-DG01
    • Amount of Enzyme
    • 20 µL each enzyme






    20 µL of each of the following enzymes in separate vials: PNGase F (Elizabethkingia meningosepticum), O-Glycosidase (Streptococcus pneumoniae), Sialidase (Arthrobacter ureafaciens), β-Galactosidase (Streptococcus pneumoniae), Glucosaminidase (Streptococcus pneumonia).


    Kit includes enzyme, reaction buffers and Bovine Fetuin (control).

    Each enzyme sufficient for up to 20 reactions.



  • Enzymatic DeGlycoMx Kit



    Ludger Enzymatic Release enzyme structure

    KE-DGMX

    This kit developed for protein deglycosylation includes our DeGlycoMx, a premixed cocktail of the enzymes required to remove all N-linked oligosaccharides and most O-linked sugars from 0.5 mg of glycoprotein, via 10 reactions of up to 50 micrograms of protein per reaction.

    The kit is easy-to-use and effective: add 2 µL of the DeGlycoMx enzyme to your denatured sample and the included buffer and incubate for 3 hours at 37°C.


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • KE-DGMX
    • Amount of Enzyme
    • 20 µL premix






    The enzymes are provided as one 20 µL premixed cocktail including: PNGase F (Elizabethkingia meningosepticum), O-Glycosidase (Streptococcus pneumoniae), Sialidase (Arthrobacter ureafaciens), β-Galactosidase (Streptococcus pneumoniae), Glucosaminidase (Streptococcus pneumoniae).


    Kit includes enzyme plus reaction buffers.

    Sufficient for up to 20 reactions.



  • Endo-β-Galactosidase



    Ludger Endo-beta-galactosidase enzyme structure

    E-XBG01

    Endo-β-galactosidase cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures.

    Recombinant gene from Bacteroides fragilis in E. Coli.


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-XBG01
    • Amount of Enzyme
    • 0.9 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • LudgerZyme Ceramide Glycanase Kit



    Ludger Ceramide Glycanase enzyme structure

    LZ-CER-HM-KIT

    Ceramide glycanase deglycosylates a variety of glycosphingolipids by cleaving the ß-glycosyl linkage.

    From Hirudo medicinalis


    Product overview

    Product guide

    Certificate of stability

    CofA:   Batch # B7A3-01   Batch # B918-03   Batch # B95G-03

    • Part Number
    • LZ-CER-HM-KIT
    • Amount of Enzyme
    • 55 µL






    Kit includes enzyme, reaction buffer and GM1 standard.

    Sufficient for up to 25 reactions.



Companion Products


Glycan labelling kits
Ludger Glycan Labelling Summary Table
Check Ludger glycan labelling technology


Post-endoglycosidase glycan clean-up with PBM 96 well plate
Ludger Glycan Clean Up Summary Table
Check Ludger glycan clean up technology


Process controls for endoglycosidase digestions: Glycoprotein (GCP) standards
GCP-IGG and GCP-FETUIN

Ludger Glycoprotein Standards
Check Ludger glycoprotein standards technology