N-Glycan Release & 2-AB Velocity Labelling Module

Product Code: LT-KAB-VP30-MOD
Size 30 samples

£1,800

Qty

This 2-AB labelling bundle kit includes PNGase F enzyme for in-solution release of N-glycans, 2-AB labelling kit and clean-up cartridges for post-labelling clean-up. The kit also includes an IgG glycoprotein standard to be used as a process control as well as a glucose homopolymer (system suitability standard). The protocol presented is the golden standard methodology for the analysis of N-linked glycans using (U)HPLC, LC-MS or MALDI-MS approaches.

This bundle kit includes reagents sufficient for the analysis of 30 samples.

Product guide

Product Specifications

Application: Release of N-glycans from glycoproteins, subsequent fluorophore labelling and clean up.

Description: This kit contains the reagents needed for PNGase F release of glycoproteins, followed by fluorophore labelling and the solid phase extraction (SPE) cartridges required for the purification of the labelled glycans.

Number of Samples: 30 separate analytical samples per kit.

Amount of Sample: As a guideline up to 50 to 100 μg of glycoprotein per sample for release using PNGase F enzyme.

Suitable Samples: Glycoproteins containing N-linked glycans.

Storage:

Product code	Stability	Storage upon arrival
Main components
LZ-rPNGaseF-30	2 years	4°C
LT-KAB-VP24	3 years	Room temperature
LC-S-A6	10 years	Room temperature
Process control
GCP-IGG-100U	5 years	-20°C
System suitability control
CAB-GHP-30	5 years	-20°C

Shipping: The product should be shipped at 4°C.

Handling: Ensure that any glass, plasticware or solvents used with this item are free of environmental carbohydrates and contaminating enzymes. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrates.

Safety: For research use only. Not for human or drug use. Please read the Safety Data Sheets (SDSs) for all chemicals used. All processes involving labelling reagents should be performed using appropriate personal safety protection – safety glasses, chemically resistant gloves (e.g. nitrile), lab coat, and when appropriate, in a laboratory fume cupboard.

Kit Contents:

Product	Product code	Quantity	Components
Main components
PNGase F release kit	LZ-rPNGaseF-30	1	PNGase F enzyme
			Reaction buffer
			NP-40 surfactant
			Denaturant
2-AB labelling kit with picoline borane	LT-KAB-VP24	1	2-AB dye
			Picoline borane reductant
			Acetic acid - DMSO
LudgerClean S cartridges	LC-S-A6	5	S cartridges

Process control
Human IgG Glycoprotein Standard	GCP-IGG-100U	2	IgG glycoprotein standard

System suitability control
2-AB labelled glucose homopolymer ladder	CAB-GHP-30	1	2-AB GHP ladder