This 2-AB labelling bundle kit includes PNGase F enzyme for in-solution release of N-glycans, 2-AB labelling kit and clean-up cartridges for post-labelling clean-up. The kit also includes an IgG glycoprotein standard to be used as a process control as well as a glucose homopolymer (system suitability standard). The protocol presented is the golden standard methodology for the analysis of N-linked glycans using (U)HPLC, LC-MS or MALDI-MS approaches.