Ludger V-Tag Glycan Release and Labelling Kit

Our V-Tag glycan kit enables release of N-glycans from glycoproteins, N-glycan labelling and clean up within 1 hour for a single IgG type sample and 2 hours for 30 IgG type samples (timing for release by PNGase F might need to be adjusted for complex type samples). The glycans are then ready for analysis by (U)HPLC. Also, once labeled with V-Tag the glycans can be incubated with exoglycosidases to allow for structure assignment.

Each kit contains PNGase F, V-Tag fluorophore dye, reagents and SPE cartridges, sufficient for up to 30 samples.

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Workflow:

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Presentation Quick Links:

1. Ludger V-Tag for N-glycan profiling and identification
2. Highlights of the V-Tag System
3. Validation Data
4. Exoglycosidase Digestion Data
5. Next Steps

 Downloadable pdf version of presentation

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View product documentation

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Product guide

Compiled Safety Data Sheets (SDS)

Specifications for LT-VTAG-C30

Application: Release of aminoglycans from glycoproteins, subsequent fluorophore labeling and clean up

Description: This kit contains the reagents needed for PNGase F release of aminoglycans, followed by fluorophore labeling and the specialised solid phase extraction (SPE) cartridges required for the purification of the labeled glycans.

Number of Samples: 30 separate analytical samples per kit.

Amount of Sample: As a guideline up to 100 μg of glycoprotein per sample.

Suitable Samples: Glycoproteins and glycopeptides containing N-linked glycans.

Storage: Store at 4°C. Protect from sources of heat and light.

Shipping: The product should be shipped at 4°C.

Handling: Ensure that any glass, plastic ware or solvents used with this item are free of environmental carbohydrates and contaminating enzymes. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety: For research use only. Not for human or drug use . Please read the Safety Data Sheets (SDS's) for all chemicals used. All processes involving labeling reagents should be performed using appropriate personal safety protection – safety glasses, chemically resistant gloves (e.g. nitrile), lab coat, and when appropriate, in a laboratory fume cupboard.

Kit Contents

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Handling: Ensure that any glass, plastic ware or solvents used with this item are free of environmental carbohydrates and contaminating enzymes. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety: For research use only. Not for human or drug use Please read the Safety Data Sheets (SDS's) for all chemicals used. All processes involving labeling reagents should be performed using appropriate personal safety protection – safety glasses, chemically resistant gloves (e.g. nitrile), lab coat, and when appropriate, in a laboratory fume cupboard.

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Each labeling reaction set consists of one vial of each of the following: