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NGA2F glycan (FA2, G0F)

NGA2F glycan (FA2, G0F)

NGA2F glycan (FA2, G0F)

  • Product Code: CN-NGA2F-20U
  • Size 20 µg
  • £530

Biantennary N-glycan that contains terminal N-acetylglucosamine residues, and fucosylation on the core GlcNAc. (size: 20µg)  m/z: 1462.5444

NGA2F: Asialo-, agalacto-, core-fucosylated bi-antennary complex-type N-glycan (oligosaccharide). NGA2F is the agalacto- substructure of NA2F (G2F) glycan and is found on many mammalian glycoproteins including human IgG and is a substructure of bi-antennary N-linked oligosaccharides such as A2F, A1F, and NA2F which are widely found on glycoproteins. This product is typically purified from the oligosaccharide pool released from porcine serum usually by enzymatic release (but also chemical hydrolysis) using a combination of HPLC and glycosidase digestion. NGA2F can also be directly released from Human IgG using HPLC without any glycosidase digestion.

Product Specification

G0F Glycan (NGA2F) Synonyms NGA2F N-linked oligosaccharide, F(6)A2

Description: G0F: Asialo-, agalacto-, core-fucosylated bi-antennary complex-type N-glycan (oligosaccharide). NGA2F is the agalacto- substructure of NA2F (G2F) glycan.

Molecular Weight: 1463

Purity: >90% pure as assessed by a combination of 1 H-NMR and HPLC

Sources: The NGA2F (G0F) glycan is found on many mammalian glycoproteins including human IgG and is a naturally occurring oligosaccharide or a substructure of bi-antennary N-linked oligosaccharides such as A2F, A1F, and NA2F which are also widely found on glycoproteins. This product is typically purified from the oligosaccharide pool released from porcine serum usually by enzymatic release (but also chemical hydrolysis) using a combination of HPLC and glycosidase digestion. NGA2F can also be directly released from Human IgG using HPLC without any glycosidase digestion.

Form: Dry. Dried by centrifugal evaporation from an aqueous solution. No salts.

Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.

Shipping: The product can be shipped at ambient when dry. After dissolution, ship on dry ice.

Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharides into the solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates—Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.

Safety: This product is non-hazardous and purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.

HPLC Analysis of Glycans

LudgerPure unlabeled glycans and LudgerTag labelled glycans may be separated and analysed by various HPLC (high-pressure liquid chromatography) methods using LudgerSep™ HPLC columns.

The LudgerSep columns are available for the following applications:

Separation of charged and neutral glycans via anion exchange columns:
LS-C3-7.5×75 LudgerSep C3 – 7.5x75mm
LS-C2-4.6×50 LudgerSep C2 – 4.6x50mm

Profile analysis of neutral and charged glycans via normal phase columns:
LS-N2-2.0×250 LudgerSep N2 – 2.00x250mm
LS-N2-4.6×250 LudgerSep N2 – 4.6x250mm
LS-N1-4.6×250 LudgerSep N1

The LudgerSep N2 columns are an especially powerful tool for the purification and analysis of LudgerTag-labelled oligosaccharides from complex glycan mixtures. Please contact us for advice regarding your particular application.

Mass Spectrometry and Electrophoresis

LudgerPure and LudgerTag labelled glycans may also be analysed by mass spectrometry, electrophoresis, and various types of spectroscopy. Please call us for advice on the dyes and analysis conditions most suitable for your intended analyses.