Fetuin glycoprotein standard

Fetuin glycoprotein standard


  • Product Code: GCP-FET-05
  • Size 500 µg

  • £46

The Fetuin Glycoprotein Standard is a highly sialylated glycoprotein standard which can be used as a positive control during glycan release and labelling (particularly for DMB sialic acid analysis). Fetuin exists in a variety of glycoforms containing bi-, tri-, and tetraantennary N-linked oligosaccharides with variable sialylation, as well as O-linked glycans.

1 vial containing 0.5 mg fetuin glycoprotein

View Product documentation


SDS

Product Specification


The fetuin glycoprotein standard was developed for use during glycan release and labeling(particularly for DMB sialic acid analysis). Fetuin exists in a variety of glycoforms containing bi-, tri-, and tetra-antennary oligosaccharides with variable sialylation, as well as O-linked glycans.

Amount of Glycoprotein Supplied
GCP-FET-05         500 µg
GCP-FET-250U     250 µg
GCP-FET-50Ux4     50 µg per vial – 4 vials

Source Fetal calf serum

Form Dry. Lyophilised powder.

Molecular Weight 36 kDa (protein weight only)

Amount of Protein
GCP-FET-05         300 µg
GCP-FET-250U     150 µg
GCP-FET-50Ux4     32 µg per vial – 4 vials
In comparison to BSA standard, determined by BCA assay. Value rounded to nearest μg.

Analysis
Fetuin glycans were released from the Fetuin Glycoprotein Standard (Cat# GCP-FET-05) using PNGase-F.

Following release the glycans were labeled using 2-Aminobenzamide (2-AB) using the LudgerTag 2-AB Glycan Labeling Kit (Cat# LT-KAB-A2).

Figure 1 shows a LudgerSep N2 HPLC profile of bovine fetuin N-glycans. To thoroughly investigate the N-glycans we first separate them based on charge on a LudgerSep C3 column (Figure 2) and then run each fraction on a LudgerSep N2 column.

Storage Refrigerate (-20°C) both before and after dissolving. This product is stable for at least 5 years as supplied.

Shipping The product is shipped at ambient temperature.

Handling Once dissolved avoid repeated thawing and refreezing, storage over 3 hours at room temperature or above, exposure to light and long term exposure to acid as these will cause glycan desialylation.

Safety This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.