LudgerSep uR2 UHPLC column - monosaccharide

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Product guide

 SDS

Specifications for LudgerSep™ uR2 Column

Applications: Analysis of monosaccharides labeled with 2-aminobenzoic acid (2-AA) using UHPLC.

Description: The LudgerSep™ uR2 UHPLC column contains particles with an endcapped octadecylsilane coating optimized for hydrophobic chromatography.

Particles: 1.9 μm silica derivatized with octadecylsiliane coating. 175 Angstrom pore size.

Cat #	Diameter x Length	Column Volume
LS-UR2-2.1x50	2.1 x 50 mm	0.17 ml

Column Tube: Stainless steel

Flow Rates: Typical flow rates = 0.1 – 1.0 ml/min.

Pressure: Pressure should not exceed 15000psi.

pH Range: 1 - 11

Temperature: Typical operating temperature = 35 oC, but increasing the temperature may improve resolution for some samples.
Maximum temperature range = 15 - 60 oC.

Solvents: Solvent A: 0.2% butylamine/ 0.5% phosphoric acid/ 1% tetrahydrofuran in purified water (A pre-prepared x10 concentrate of this solvent is available from Ludger, catalogue number LS-R-BPTX10).
Solvent B: Acetonitrile
Avoid strong oxidants and anionic detergents.

Column Protection: Filter all solvents to 0.2 μm and degas using either helium sparging or vacuum degassing.
Filter all samples using a 0.2 μm filter membrane before loading onto the column.Install a good quality in-line filter between the sample injector and the column. Please call us for advice on the most suitable sample and in-line filters to use. Long term storage of the column should be in solvent containing at least 30% acetonitrile.

Suitable Samples: Monosaccharides labeled with 2-aminobenzoic acid (2-AA)

Sample : Filter samples to 0.2 m and avoid exposure to light. For best resolution of chromatogram peaks use a full loop injection method with a sample loop of 5 μl volume or less.

Sample Detection: Fluorescence. Excitation: 360 nm. Emission: 425 nm.

Handling: Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety: Please read the Material Safety Data Sheets (MSDS's) for all chemicals used. All processes involving labelling reagents should be performed using appropriate personal safety protection - eyeglasses, chemically resistant gloves (e.g. nitrile), etc. - and where appropriate in a laboratory fume cupboard

For research use only. Not for human or drug use

HPLC System Requirements
The LudgerSep™ uR2 column is designed for use with the latest generation of UHPLC instruments capable of withstanding high flow pressures and fast sample analyses. In order to take advantage of the high resolving power of sub 3 μm particle size containing columns, we recommend keeping sample injection volumes below 5 μL and minimising system void volumes. Ideally use full loop injection with a sample loop of less than 5 μL. Tubing should be narrow bore (about 0.13 mm diameter or less) and detector flow cell volumes should be 10 μl or less. Although an example chromatogram is shown in this guide, retention times will vary dependent on the UHPLC system used.