LudgerClean CEX cartridges for O-glycan purification

Product Code: LC-CEX-A6
Size 6 cartridges

£183

Qty

Cation exchange resin cartridges. For purification of O-glycans. Each pack contains 6 cartridges.

LC-CEX-A6 are used for purification of O-glycans from non-carbohydrate material including salts, proteins, and detergents. Applications include the cleanup of glycans following hydrazinolysis or any other non-reductive O-glycan release method.

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Product guide

Specifications for LC-CEX-A6 Application

CEX cartridges were designed for the purification of O-glycans from non-carbohydrate material including salts, proteins, and detergents. Applications include cleanup of glycans following hydrazinolysis or any other non-reductive O-glycan release method.

Description The cartridges contain a cation exchange resin that is stored in 1 M hydrochloric acid.

Binding capacity Each LudgerClean CEX cartridge can typically clean up O-glycans released from 1000 µg of glycoprotein.

Number of Samples LudgerClean CEX cartridges are designed for single use only.

Suitable Samples A wide range of O-glycans can be purified including di-saccharides, tri-saccharides and oligosaccharides. Glycan samples must be applied to the cartridges in solutions that are substantially aqueous.

Structural Integrity No detectable loss of sialylated glycans.

Binding Selectivity Essentially stoichiometric binding and elution for most complex glycan mixtures.

Storage Store refrigerated at 4˚C in the dark. Protect from sources of heat, light, and moisture. The cartridges are stable for at least 6 months from date of manufacture.

Shipping: The product can be shipped at ambient temperature.

Handling: Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety: Please read the Material Safety Data Sheets (MSDS's) for all chemicals used. All processes involving hazardous reagents should be performed using appropriate personal safety protection - eyeglasses, chemically resistant gloves (e.g. nitrile), etc. - and where appropriate in a laboratory fume cupboard.

For research use only. Not for human or drug use

• Pure water: resistivity 18 MΩ-cm, particle free (>0.22 μm), TOC <10 ppb
• Pipettes
• pH paper
• Centrifugal evaporator (e.g.ThermoSavant SpeedVac® or GeneVac®).